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Analítica de triglicéridos por cromatografía líquida de alta eficacia: aplicaciones a aceites de origen vegetal

Authors: Castellote Bargalló, Ana Isabel;

Analítica de triglicéridos por cromatografía líquida de alta eficacia: aplicaciones a aceites de origen vegetal

Abstract

[spa] Dado el gran interés que despierta dentro del campo del análisis de lípidos la determinación de la composición triglicérica y por lo tanto el disponer de un método aplicable al análisis rutinario, se han puesto a punto dos técnicas por cromatografía liquida de alta eficacia (CLAE) para el análisis de triglicéridos que utilizan dos sistemas de detección distintos: detector de índice de refracción (IR) y detector UV a longitudes de onda bajas. Una vez optimizados los parámetros cromatográficos y estudiado los aspectos cuantitativos de ambas técnicas (linealidad de respuesta, límites de detección cuali y cuantitativos, factores de respuesta, repetibilidad y reproductibilidad) se comparan ambas, eligiéndose la CLAE-IR como la mas óptima. Esta técnica se aplica primero al estudio cualitativo de los triglicéridos de los aceites de oliva, soja, girasol, maíz, coco y palma, caracterizándolos por sus perfiles cromatográficos. Seguidamente, sobre un total de 138 muestras, se aplica la técnica al estudio cuantitativo de los triglicéridos del aceite de oliva, tanto para su caracterización (estableciendo los valores medios de los triglicéridos en los aceites vírgenes) como para la diferenciación entre los cuatro tipos que contempla el C.A.E.: aceites vírgenes, puros, refinados y de orujo de aceituna. Asimismo se establecen subgrupos dentro de los aceites vírgenes según su procedencia: Andalucía, País Valenciano y Cataluña, y dentro de esta última, también según sus dos denominaciones de origen (D.O. Borges Blanques y D.O. Ciurana) para proceder también a su posterior diferenciación. Finalmente se hace un estudio de las posibilidades de la aplicación de la técnica de cromatografía liquida de alta eficacia con detector de IR a la detección de fraudes de aceites de oliva con aceites de semillas a través de las variaciones de su composición triglicérica.

Country
Spain
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Keywords

Oils and fats, 663/664, Olis i greixos, Liquid chromatography, Aceites y grasas, Cromatografía líquida, Cromatografia de líquids, Ciències Experimentals i Matemàtiques

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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