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LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas
Article . 2013
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LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas
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Cryoletters
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Cryopreservation of Arachis pintoi (leguminosae) somatic embryos.

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Jan 2013 Argentina, France English Publisher:Cryo LettersJournal:Cryo letters, volume 34 (issn: 0143-2044, Copyright policy )
Authors: Rey, H.Y.; Faloci, M.; Medina, R.; Dolce, N.; /Engelmann, Florent; Mroginski, L.;

pmid: 24441368

handle: 11336/2255

Cryopreservation of Arachis pintoi (leguminosae) somatic embryos.

Abstract

In this study, we successfully cryopreserved cotyledonary somatic embryos of diploid and triploid Arachis pintoi cytotypes using the encapsulation-dehydration technique. The highest survival rates were obtained when somatic embryos were encapsulated in calcium alginate beads and precultured in agitated (80 rpm) liquid establishment medium (EM) with daily increasing sucrose concentration (0.50, 0.75, and 1.0 M). The encapsulated somatic embryos were then dehydrated with silica gel for 5 h to 20% moisture content (fresh weight basis) and cooled either rapidly (direct immersion in liquid nitrogen, LN) or slowly (1ºC min-1 from 25ºC to -30ºC followed by immersion in LN). Beads were kept in LN for a minimum of 1 h and then were rapidly rewarmed in a 30ºC water-bath for 2 min. Finally, encapsulated somatic embryos were post-cultured in agitated (80 rpm) liquid EM with daily decreasing sucrose concentration (0.75 and 0.5 M) and transferred to solidified EM. Using this protocol, we obtained 26% and 30% plant regeneration from cryopreserved somatic embryos of diploid and triploid cytotypes. No morphological abnormalities were observed in any of the plants regenerated from cryopreserved embryos and their genetic stability was confirmed with 10 isozyme systems and nine RAPD profiles.

Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.

Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.

Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.

Fil: Medina, Ricardo D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina.

Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche Pour Le Developpement; Francia.

Fil: Rey, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica; Argentina.

Fil: Dolce, Natalia Raquel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.

Fil: Mroginski, Luis Amado. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.

Fil: Faloci, Mirta Mabel. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina.

Countries
Argentina, France
Related Organizations
Keywords

Arachis, ISOENZYME, Alginates, 630, DESHYDRATATION, Glucuronic Acid, https://purl.org/becyt/ford/1.6, ETUDE COMPARATIVE, ENCAPSULATION, Desiccation, Genetic Stability, https://purl.org/becyt/ford/1, SUCROSE, ANALYSE STATISTIQUE, Encapsulation-Dehydration, Cryopreservation, EXPERIMENTATION IN VITRO, Hexuronic Acids, LEGUMINEUSE, Somatic embryo, EMBRYOGENESE SOMATIQUE, TECHNIQUE RAPD, Genetic stability, CRYOPRESERVATION, Silicon Dioxide, CONSERVATION DES RESSOURCES GENETIQUES, STABILITE GENETIQUE, Somatic Embryo, ETUDE EXPERIMENTALE, Encapsulation-dehydration

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    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
Green