[Inhibition of proliferation of H5N1 subtype AIV in CEF by chemosynthetic siRNA].
Copyright policy )[Inhibition of proliferation of H5N1 subtype AIV in CEF by chemosynthetic siRNA].
In order to study the proliferation inhibition effect of H5N1 subtype avian influenza virus (AIV) with small interfere RNA (siRNA), a total of 4 siRNAs were designed in accordance with the NP and PA genes of H5N1 subtype AIV, the siRNAs were then transfected to chicken embryo fibroblast(CEF), CEF was infected with H5N1 subtype AIV after 6 hrs. Virus titer of cell supernatant was tested at 16-56hrs post infection, and pathological changes of the cells was observed; mRNA levels of NP, PA, HA and p13-actin gene were tested at 36hrs post infection. The results showed that these 4 siRNAs could inhibit the prolif-eration of H5N1 subtype AIV in CEF in varying degrees, and one siRNA targeting PA was best per-formed. The experimental results also showed that the inhibition effect was decreased with the time prolonged. This research provides a basis for further studying RNAi on AIV prevention and control.
Influenza A Virus, H5N1 Subtype, Hemagglutination, Viral Core Proteins, RNA-Binding Proteins, Hemagglutinin Glycoproteins, Influenza Virus, Chick Embryo, Fibroblasts, Nucleocapsid Proteins, RNA-Dependent RNA Polymerase, Real-Time Polymerase Chain Reaction, Transfection, Actins, Specific Pathogen-Free Organisms, Viral Proteins, Hemagglutinins, Animals, Humans, RNA Interference, RNA, Small Interfering, DNA Primers
Influenza A Virus, H5N1 Subtype, Hemagglutination, Viral Core Proteins, RNA-Binding Proteins, Hemagglutinin Glycoproteins, Influenza Virus, Chick Embryo, Fibroblasts, Nucleocapsid Proteins, RNA-Dependent RNA Polymerase, Real-Time Polymerase Chain Reaction, Transfection, Actins, Specific Pathogen-Free Organisms, Viral Proteins, Hemagglutinins, Animals, Humans, RNA Interference, RNA, Small Interfering, DNA Primers
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