Rhizobial lipopolysaccharide as the receptor in lectin-Rhizobium interaction.
Copyright policy )Rhizobial lipopolysaccharide as the receptor in lectin-Rhizobium interaction.
Rhizobial specificity was examined on the basis of interaction between legume lectins (peanut, pea and soybean) and different rhizobial species (various bradyrhizobia specific for peanut, P 14-93 and SB16). Legume lectins showed higher affinity towards host-specific Rhizobium and lipopolysaccharides (LPS) isolated from those particular rhizobia. Two LPS mutants of peanut-specific Bradyrhizobiumn sp. (Arachis) strain GN17 were isolated by Tn5 mutagenesis. These mutants (GN17M1 and GN17M2) were characterized by their higher hydrophobicity with respect to the parent cells. The hexose content in exopolysaccharides (EPS) and LPS of the mutants was found reduced significantly, whereas 2-keto-3-deoxyoctulosonic acid (Kdo) and uronic acid in LPS were less by 20-times and thrice, respectively in the mutants. Glucose was the major sugar in LPS from all the strains. However, glucosamine appeared only in the mutants. Spectrofluorimetric analysis showed that LPS from GN17M1 mutant interacted most significantly with peanut root agglutinin or lectin (PRA II). The results indicate that LPS on the surface of rhizobial cells is the possible receptor for lectin.
Lipopolysaccharides, Arachis, Glycine max, Cell Membrane, Carbohydrates, Peanut Agglutinin, Spectrometry, Fluorescence, Mutagenesis, Polysaccharides, Lectins, Mutation, Biotinylation, Plant Lectins, Protein Binding, Rhizobium
Lipopolysaccharides, Arachis, Glycine max, Cell Membrane, Carbohydrates, Peanut Agglutinin, Spectrometry, Fluorescence, Mutagenesis, Polysaccharides, Lectins, Mutation, Biotinylation, Plant Lectins, Protein Binding, Rhizobium
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).3 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!