To establish the seedling quality grading standard of Dendrobium nobile.Height, stem thickness and stem nodes of seedlings (test-tube seedling and cutting seedling) from Chishui city Guizhou province were tested. Through statistical analysis, the key indicators for seedling quality grading were defined.Height and the number of cluster were the primary indicator of test-tube seedling; Height and the number of buds were the primary indicator of cutting seedling.The grade test-tube seedling quality of each grade should reach the following requirements: for the first grade seedlings height > or = 17cm, the numbers of cluster > or = 3; For the second seedling height 11 -17cm, the numbers of cluster > or =2. The grade cutting seedling requires the first grade seedlings height > or = 12cm, the numbers of buds > or = 3; The second grade seedling height 6 - 12cm, the numbers of cluster > or = 2. Seedlings those can hot reach the second grade requirements are defined as unqualified seedlings.