[ELISA test-kit for herpes 2 serodiagnosis].

H V, Kovtoniuk; L M, Korshun; V O, Shevchuk; O K, Kysel'ova; M I, Vudmaska; L O, Hanova; M I, Tereshchenko; L M, Moĭsa; M Ia, Spivak

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Mikrobiolohichnyi Zhurnal

Article . 2012

Data sources: Europe PubMed Central

[ELISA test-kit for herpes 2 serodiagnosis].

descriptionPublicationkeyboard_double_arrow_right Article 26 Jun 2012Journal:Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993), volume 74 (issn: 1028-0987,

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Authors: H V, Kovtoniuk; L M, Korshun; V O, Shevchuk; O K, Kysel'ova; M I, Vudmaska; L O, Hanova; M I, Tereshchenko; +2 Authors

pmid: 22686021

[ELISA test-kit for herpes 2 serodiagnosis].

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Abstract

To reveal Herpes simplex virus 2 specific IgG and to determine their avidity the ELISA test-kit was constructed using recombinant protein gG2 (PSC SPC Diaproph-Med). Using this test-kit the distribution of specific antibodies with different avidity indexes was investigated in practically healthy donor samples. It is possible to use the mentioned ELISA test-kit for confirmation of primary infection, and also for differentiation of primary infection, chronic disease and virus reactivation. Thus, this ELISA test-kit could be an additional tool in herpetic infection diagnosis.

Keywords

Herpes Genitalis, Herpesvirus 2, Human, Antibody Affinity, Enzyme-Linked Immunosorbent Assay, Antibodies, Viral, Severity of Illness Index, Recombinant Proteins, Immunoglobulin G, Chronic Disease, Escherichia coli, Humans, Virus Activation, Reagent Kits, Diagnostic, Cloning, Molecular, Plasmids

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