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Effect of culture media substitutions on in vitro growth of Ipecac

Authors: Jiménez-Rivera, Alexander; Montero-Carmona, Wayner;

Effect of culture media substitutions on in vitro growth of Ipecac

Abstract

La micropropagación de raicilla se ha visto como una alternativa para suplir la necesidad de materiales para productores interesados en el cultivo. No obstante, los costos poco competitivos del cultivo de tejidos tradicional han dificultado su implementación en la producción de vitroplantas para zonas de cultivo. Con el fin de evaluar diferentes sustituciones en la composición del medio de cultivo, se realizó una comparación del crecimiento de plantas en medios con sustituciones en las sales minerales (por sales hidropónicas), Vitaminas (por tiamina o complejo B1, B6 y B12) y gelificante (por almidón de maíz). Se observaron diferencias significativas (a=0,05) entre las características de crecimiento evaluadas (número de brotes, longitud de brotes, número de hojas y peso seco). Un análisis multivariado mostró una relación entre los medios con sustitución de sales hidropónicas, los medios con vitaminas (B1, B6 y B12) y el medio Testigo (MS); los cuales influenciaron la longitud de brotes y el número de hojas. Por su parte el medio con sustitución de almidón de maíz afectó el número de brotes y el peso seco. Esta última variable también se vio influenciada por el medio de Bajo Costo (sustitución de los tres componentes). De los tratamientos evaluados, el medio con sustitución de Tiamina fue el que presentó los rendimientos más bajos; mientras el medio de Bajo Costo los más elevados según Prueba de Hotelling - Bonferroni. El análisis de costos realizado mostró un ahorro significativo (hasta 59%) entre el medio de Bajo Costo y el tratamiento testigo (Medio MS).

The micropropagation of ipecac has been seen as an alternative to supply the need for materials from producers interested in this crop. However, the uncompetitive costs of traditional tissue culture have hampered their implementation in the production of vitroplants for growing areas. In order to evaluate different substitutions in the composition of the culture media, a comparison on plants growth in different media with substitutions in mineral salts (by hydroponic salts), vitamins (by thiamine or complex B1, B6 and B12) and gelling agent (by corn starch) was made. Significant differences (a = 0.05) between the evaluated growth characteristics (shoots number, shoots length, leaves number and dry weight) were observed. A multivariate analysis showed a relationship between culture media with hydroponic salts substitution, with vitamins (B1, B6 and B12) substitution and the control media (MS); which influenced the shoots length and the leaves number. On the other hand, the media with corn starch substitution affected the shoots number and the dry weight. This last variable was also influenced by the Low Cost media (substitution of the three components). Of all treatments evaluated, the Thiamine substitution media was the one that presented the lowest yields; while the Low Cost media shows the highest according to Hotelling - Bonferroni Test. The cost analysis performed showed significant savings (up to 59%) between the Low Cost and the control treatment (MS media).

Country
Costa Rica
Related Organizations
Keywords

micropropagation, micropropagación, Medios de bajo costo, Psychotria ipecacuanha, Psychotria ipecacuanha, nudos, nodal segment, Low cost culture media

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green