To compare volatile oil of Lonicera confusa (red gland Lonicera) fresh leaves with dry leaves and provide evidence for further study of Lonicera confusa leaves.GC-MS was used to identify chemical composition in volatile oil in red gland Lonicera leaves.93 chemical components were separated in fresh leaves in which 39 chemical components were identified; 88 chemical components were separated in dry leaves in which 51 chemical components were identified. The main components of fresh leaves of red gland Lonicera were n-Hexadecanoic acid (11.90%), Phytol (11.79) and 9,12,15-Octadecatrienoic acid, methyl ester, (Z, Z, Z)(7.08); The main components of dry leaves of red gland Lonicera were 1,6-Octadien-3-ol, 3,7-dimethyl-(27.62%), Phytol (7.57%) and 2, 6, 10, 14, 18, 22-Tetracosahexaene, 2, 6, 10, 15, 19, 23-hexamethyl-, (all-E) (4.70%).There are significant differences between volatile oil between fresh and dry leaves.