Cissus populnea has been used locally to treat many ailments such as venereal, stomach and skin infections; and also used as laxative or purgative. Economically it has been used as binder in food and in lining dye pits. This work aims at determining the type of anthraquinones from the stem bark of C. populnea which might be a potential source of drugs (laxative/cathartic) using thin layer chramatograpy (TLC) and senna leaf as reference. The analysis showed the stem bark anthroquinone extract to contain physcion and chrysophanol.