Detection by PCR the frequency of clbB, clbN, clbA H clbQ genes of Pks-pathogenicity island in clinical strains ofenterobacteria.112 strains various genera and species of enterobacteria, including 16 museum and 96 clinical are investigated. Isolated strains represents Escherichia species (n = 68), Klebsiella (n = 16), Enterobacter (n = 9), Serratia (n = 7) and others minor species of Enterobacteriaceae family (n = 12). Fifty nine strains isolated from urine of urinary tract infection, 26 isolates from intestines of patients with dysbiosis and 11--from children with complications after a liver transplantation. A total bacterial isolates were screened by multiplex PCRforthe presence ofclbB, clbN, clbA and clbQ genes.Among 41 uropathogenic E.coli it is revealed 15 (36,6%) Pks-positive strains carring all of clbB, clbN, clbA ? clbQ genes, that composed 27,1% from total number of the enterobacteria, isolates from urine. Among 44 clinical isolates of various species of enterobacteria only one Pks-positive strain K. pneumoniae was revealed. Strains enterobacteria, isolated at pyoinflammatory complications after liver transplantation (n = 11) and isolates from intestinal tract in dysbiosis (n = 26), were Pks-negative.The clbB, clbN, clbA ? clbQ genes of the Pks-island which have been detected in 36,6 % E. coli urological strains are markers of pathogenicity of clinical isolates of extraintestinal origin and advisable of their detection by PCR.