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Variación de la microbiota oral por factores ambientales

Authors: Encina Santiso, Juan;

Variación de la microbiota oral por factores ambientales

Abstract

[Resumen]En este trabajo se ha estudiado cómo influye fumar, beber alcohol, cepillarse los dientes, morderse las uñas y llevar ortodoncia en las comunidades microbianas de la boca. Para ello, se tomaron veintidós muestras de la boca de sus correspondientes voluntarias y se realizaron con ellas varias diluciones seriadas que fueron sembradas por triplicado en tres medios sólidos diferentes en placas de Petri: tripticasa de soja, VRBG y Mueller-Hinton con sangre de cordero al 5%. Mediante el cultivo en aerobiosis a 37ºC durante 24-72 h se obtuvo información de la carga total de bacterias mesófilas aerobias, de bacterias hemolíticas y de bacterias fecales presentes en la cavidad oral. Como resultado del recuento de colonias se ha obtenido que llevar ortodoncia incrementa sustancialmente la carga microbiana total de la boca y de las bacterias α-hemolíticas en particular. También se ha comprobado que morderse las uñas está directamente relacionado con el aislamiento de bacterias fecales en la cavidad oral, que son eliminadas por el consumo de drogas legales (alcohol y tabaco). No se ha detectado efectos en cuanto a los niveles de cepillado.

[Resumo] Neste traballo estudouse cómo inflúe fumar, beber alcohol, cepillarse os dentes, morderse as unllas e levar ortodoncia nas comunidades microbianas da boca. Para iso, tomáronse vinte e dúas mostras da boca das súas correspondentes voluntarias e realizáronse con elas varias dilucións seriadas que foron sementadas por triplicado en tres medios sólidos diferentes en placas de Petri: tripticasa de soia, VRBG e Mueller-Hinton con sangue de año ao 5%. Mediante o cultivo en aerobiose a 37ºC durante 24-72 h obtívose información da carga total de bacterias mesófilas aerobias, de bacterias hemolíticas e de bacterias fecais presentes na cavidade oral. Como resultado do reconto de colonias obtívose que levar ortodoncia incrementa substancialmente a carga microbiana total da boca e das bacterias α-hemolíticas en particular. Tamén comprobouse que morderse as unllas está directamente relacionado co illamento de bacterias fecais na cavidade oral, que son eliminadas polo consumo de drogas legais (alcohol e tabaco). Non detectouse efectos en canto aos niveis de cepillado.

[Abstract] In this work, how smoking, drink alcohol, teeth washing, nails biting and carry orthodontics affects to microbial communities of the mouth was studied. To make that, twenty-two samples from the mouth of their respective volunteers were taken and with them some seriated dilutions were made. This dilutions were sown by triplicate in three solid media in Petri dishes: tripticase-soy, VRBG and Mueller-Hinton with lamb blood at 5%. The aerobic culture at 37ºC during 24-72 h brang us information about the total mesophilic aerobic bacteria charge, haemolytic bacteria and fecal bacteria present into oral cavity. As a result of the recount of colonies, it seems that carry orthodontics increases substantially the total microbial charge and α-haemolityc bacteria in particular. In addition, nails biting is directly linked to the isolation of fecal bacteria into oral cavity, which are eliminated by alcohol and tobacco consumption. Effects between the teeth washing levels have not been detected.

Traballo fin de grao (UDC.CIE). Bioloxía. Curso 2015/2016

Country
Spain
Related Organizations
Keywords

Ortodoncia, Tabaco, Boca, Cepillado, Uñas, Bacterias, Alcohol, Variación

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
Green