Noninvasive fetal genotyping of human platelet antigen-1a.
Copyright policy )Noninvasive fetal genotyping of human platelet antigen-1a.
We describe a reliable noninvasive fetal human platelet antigen (HPA)-1a genotyping assay on a real-time polymerase chain reaction (PCR) platform using cell-free fetal DNA isolated from maternal blood. Nonspecific amplification of maternal cell-free DNA is overcome by pre-PCR digestion of the cell-free DNA with the Msp1 restriction enzyme. Noninvasive fetal HPA-1a genotyping offers a safe method for alloimmunised pregnant women to determine whether their fetus is at risk of fetal or neonatal alloimmune thrombocytopenia (FNAIT) and whether interventions to prevent intracranial haemorrhage are required. The availability of this test is relevant to the ongoing debate on screening pregnancies for HPA-1a-mediated FNAIT.
- University of Amsterdam Netherlands
- Amsterdam UMC Netherlands
- Amsterdam UMC, location AMC Netherlands
- Leiden University Medical Center Netherlands
Genotype, fetal or neonatal alloimmune thrombocytopenia, Integrin beta3, DNA, Fetal Blood, human platelet antigen-1a, cell-free DNA, Thrombocytopenia, Neonatal Alloimmune, Pregnancy, noninvasive prenatal diagnosis, Prenatal Diagnosis, Humans, Alloimmune thrombocytopenia, maternal plasma, Antigens, Human Platelet, Female
Genotype, fetal or neonatal alloimmune thrombocytopenia, Integrin beta3, DNA, Fetal Blood, human platelet antigen-1a, cell-free DNA, Thrombocytopenia, Neonatal Alloimmune, Pregnancy, noninvasive prenatal diagnosis, Prenatal Diagnosis, Humans, Alloimmune thrombocytopenia, maternal plasma, Antigens, Human Platelet, Female
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