The aim of this study was to optimize the testing methods for seed quality, and to provide a basis for establishing seed testing rules and quality grading standard of Glycyrrhiza uralensis.Referring to the International Seed Testing Rules made by ISTA and Rules for agricultural seed testing (GB/T 3543-1995) issued by China, the seed quality of G. uralensis from different collection areas was measured.The seed testing methods for quality items of G. uralensis, including sampling, purity analysis, verification of genuineness, weight of 1 000 seeds, percentage germination, moisture content and seed viability of G. uralensis had been initially established.