The invasive Asian chestnut gall wasp (ACGW) (Dryocosmus kuriphilus Yasumatsu) is a major insect pest in chestnuts (Castanea spp.), in all its areas of distribution (6). ACGW gall formation can cause heavy reductions in plants’ growth rates and fruiting, severely damaging the chestnut market (5). Some chestnut species and hybrids have proven to be tolerant of ACGW (4) (1). This was probably due to a hypersensitive response to ACGW attack (3). Some C. sativa Mill. cultivars showed different tolerance levels to the ACGW (4). The purpose of this study is to extend current knowledge about the tolerance of C. sativa cultivars to ACGW. Methodology & Theoretical Orientation: Some C. sativa cultivars grown in the municipality of Pistoia (Tuscany, Italy) were morphologically and genetically analyzed. Current-year shoots were collected from the same trees to determine ACGW attack levels, since the number of galls per shoot is related to nut yield losses (2). Findings: Three C. sativa cultivars were identified, each showing significantly different tolerance levels to the ACGW: Carpinese, Nerattino and Pastinese. More specifically, Carpinese showed the highest level of attack, with an infested shoot rate of 84.38%, the highest number of galls per shoot, as well as bigger galls. On the contrary, Nerattino had the lowest attack rate, with only 53% of shoots affected, the lowest number of galls per shoot, and the smallest galls. Pastinese parameters were in between. Conclusion & Significance: All the cultivars studied were attacked by the ACGW, however they had different tolerances to the insect. More research on this field will help in creating a list of the more highly tolerant cultivars, which can be used for new plantations and graft nurseries