Biotinylated PAMAM dendrimers for intracellular delivery of cisplatin to ovarian cancer: role of SMVT.
Copyright policy )Biotinylated PAMAM dendrimers for intracellular delivery of cisplatin to ovarian cancer: role of SMVT.
The aim of this study was to prepare biotinylated PAMAM dendrimers loaded with cisplatin and to evaluate the cytotoxicity in ovarian cancer cell lines.Biotinylated and unconjugated dendrimer-cisplatin complexes were investigated for encapsulation efficiency, in vitro cytotoxic activity and cellular accumulation of cisplatin in OVCAR-3, SKOV-3, A2780 (wild-type) and CP70 (A2780/CP70, cisplatin-resistant) cells.Encapsulation efficiency of cisplatin ranged from 5.33% to 21.10%. In vitro cytotoxic activity revealed that IC(50) values of dendrimer-cisplatin complexes were significantly lower than that of free cisplatin in OVCAR-3, SKOV-3 and CP70 cell lines. Cellular uptake data showed highest accumulation of platinum by PAMAMG(4) NH(2) dendrimer complexes of cisplatin in A2780 (19.41±0.85 μg/ml) and CP70 (25.25±1.25 μg/ml) cell lines in comparison with cisplatin uptake of only 1.77±0.351 μg/ml in A2780 and 2.31±0.421 μg/ml in CP70 cells.In conclusion, biotinylated PAMAM dendrimers may be utilized as potential targeting agents for cisplatin delivery to ovarian cancer.
- Texas A&M Health Science Center United States
Ovarian Neoplasms, Dendrimers, Cell Death, Symporters, Cell Survival, Reverse Transcriptase Polymerase Chain Reaction, Intracellular Space, Hemolysis, Gene Expression Regulation, Neoplastic, Inhibitory Concentration 50, Drug Delivery Systems, Cell Line, Tumor, Materials Testing, Humans, Biotinylation, Female, Cisplatin, Fluorescein-5-isothiocyanate
Ovarian Neoplasms, Dendrimers, Cell Death, Symporters, Cell Survival, Reverse Transcriptase Polymerase Chain Reaction, Intracellular Space, Hemolysis, Gene Expression Regulation, Neoplastic, Inhibitory Concentration 50, Drug Delivery Systems, Cell Line, Tumor, Materials Testing, Humans, Biotinylation, Female, Cisplatin, Fluorescein-5-isothiocyanate
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