[The regulation of UTROPHIN expression by EN1].
Copyright policy [The regulation of UTROPHIN expression by EN1].
To investigate possible factors up-regulating the expression of UTROPHIN, potential regulatory elements in the promoter of the human UTROPHIN was predicted by P-match software and verified by EMSA and ChIP. The mechanism of EN1 regulation of the human UTROPHIN expression was evaluated by RNA interference and real-time PCR analyses. Two potential EN1 binding sites in UTROPHIN promoter region were predicted by P-Match software but only the second site was verified to interact directly with EN1 by EMSA and ChIP. The results from RNA interference and real-time PCR showed that the mRNA level of UTROPHIN increased in HeLa cells after EN1 was knockdowned by siRNA. It indicated that EN1 might be a negative regulatory factor for UTROPHIN. Our study suggested that UTROPHIN might be a new target for DMD therapy.
- China Medical University China (People's Republic of)
Homeodomain Proteins, Base Sequence, Gene Expression Regulation, Utrophin, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Humans, RNA Interference, HeLa Cells
Homeodomain Proteins, Base Sequence, Gene Expression Regulation, Utrophin, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Humans, RNA Interference, HeLa Cells
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