Identification of two spliced leader RNA transcripts from Perkinsus marinus.
Copyright policy )Identification of two spliced leader RNA transcripts from Perkinsus marinus.
Spliced leader (SL) variants are present in a number of mRNAs from Perkinsus marinus. Three different SLs of 22 nucleotides (nt) in length were previously reported, with a consensus sequence of (DCCGUAGCCAUYUUGGCUCAAG). A truncated 21 nt SL, with an (A) at nt-1 and a (U) deletion at nt-13, has also been reported. Here, we report an additional 21 nt SL variant with (G) at nt-1. Using cDNA analysis, a full-length SL RNA transcript was identified for both 21 nt SLs (SL2[A] and SL2[G]). This transcript is 81 nt in length and contains a conserved transcription termination sequence present in closely related dinoflagellates.
- University of Maryland Eastern Shore United States
RNA, Spliced Leader, DNA, Complementary, Base Sequence, Alveolata, Molecular Sequence Data, Gene Expression, Nucleic Acid Conformation, Sequence Analysis, DNA
RNA, Spliced Leader, DNA, Complementary, Base Sequence, Alveolata, Molecular Sequence Data, Gene Expression, Nucleic Acid Conformation, Sequence Analysis, DNA
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