[Laser nephelometry. A better methodology?].
Copyright policy )[Laser nephelometry. A better methodology?].
Nephelometric assays (LN) are known since several years ago. Laser beam immunonephelometric analysis (He-Ne: 640 nm), are a sophistication of the spectrophotometers. Nephelometry was the first method to make use of narrow-angle light scattering and a laser beam. It was made 2500 +/- 200 determinations of human fluids for IgG, IgA, IgM, C3 and C4, both LN and RID (radial immunodiffusion method). Human patients had the following characteristics: 18-55 years old, both sexes and with different pulmonary pathologies (Bronchial asthma, Bronchitis, Tuberculosis, Emphysema, Fibrosis, Extrinsic allergic alveolitis). We found a greater sensitivity and a shorter analysis time for LN than for RID. On the other hand, Nephelometry gave different values to the radial immunodiffusion method for all the proteins evaluated.
Adult, Lung Diseases, Male, Immunodiffusion, Adolescent, Immune Sera, Lasers, Immunoglobulins, Antigen-Antibody Complex, Complement System Proteins, Middle Aged, Antibodies, Anti-Idiotypic, Evaluation Studies as Topic, Nephelometry and Turbidimetry, Humans, Female
Adult, Lung Diseases, Male, Immunodiffusion, Adolescent, Immune Sera, Lasers, Immunoglobulins, Antigen-Antibody Complex, Complement System Proteins, Middle Aged, Antibodies, Anti-Idiotypic, Evaluation Studies as Topic, Nephelometry and Turbidimetry, Humans, Female
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