To facilitate the use of Biobrane for those burn care practitioners not familiar with this material.Two techniques have been developed through extensive use of Biobrane over many years, in both sheet and glove form. These techniques have been described and illustrated with photographs.The use of these techniques has allowed the corresponding author to markedly reduce operating time and to easily apply the material single-handedly.Biobrane is a biosynthetic skin substitute primarily designed for the definitive treatment of superficial partial-thickness to mid-dermal burn injury. Once experienced with its use, the material is quite ubiquitous. The described techniques will facilitate the use of Biobrane for those not familiar with it.