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Characterization of Suid herpesvirus 1 field isolates from Argentina.

Authors: Serena, Maria Soledad; Metz, German Ernesto; Martin Ocampos, Giselle Paula; Gambaro, Sabrina Eliana; Mortola, Eduardo; Echeverria, Maria Gabriela;

Characterization of Suid herpesvirus 1 field isolates from Argentina.

Abstract

Caracterización de cepas de campo de Herpesvirus suino 1 aisladas en Argentina. Se caracterizaron cepas argentinas de herpesvirus suino tipo 1 (HVS-1) mediante corte con las enzimas de restricción BamHI, BstEII y XhoI. La presencia del tipo genómico II había sido reportada en la Argentina una sola vez y, hasta el momento, no se han informado nuevos casos. Este estudio reveló una homogeneidad de los sitios BamHI, acorde con el número y el tamaño de los fragmentos. La presencia del fragmento BamHI #7 en los aislamientos argentinos analizados sugiere que éstos pertenecen al tipo salvaje (wild-type). Además, se caracterizó el principal dominio inmunogénico de la glicoproteína E de todas las cepas argentinas y se lo comparó con los de las cepas de referencia y con las secuencias disponibles en la base de datos GenBank. Los porcentajes de similitud obtenidos oscilaron entre 99 y 100%.

The genomic characterization of Suid herpesvirus 1 (SHV-1) isolates from Argentina was accomplished by restriction pattern analysis using the BamHI, BstEII and XhoI enzymes. Type II genome has been described only once in Argentina. This study revealed considerable homogeneity of BamHI endonuclease sites in all the strains analyzed, according to the number and size of the fragments. No deletion of BamHI fragment #7 among the Argentinean isolates suggests that these strains are wild-type. In addition, the main antigenic domain of glycoprotein E of all the Argentinean strains, as well as the reference strains and sequences available in the GenBank, were characterized. The similarity percent oscillated between 99 and 100%.

Fil: Martin Ocampos, Giselle Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina

Fil: Echeverria, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina

Fil: Serena, Maria Soledad. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Metz, German Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina

Fil: Gambaro, Sabrina Eliana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina

Fil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Inmunología; Argentina

Country
Argentina
Keywords

https://purl.org/becyt/ford/4.3, Argentina, Glycoprotein E, Humans, https://purl.org/becyt/ford/4, RFLP, Veterinaria, Suid herpesvirus 1, Herpesvirus 1, Suid, Suid Herpesvirus 1

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
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gold