To establish a new method for the determination of matrine in matrine injection by microfluidic chip with contactless conductivity detection.The electrophoretic parameters, such as the variety and concentration of buffer solution and additive, separation voltage and injection time, etc. were researched.In the optimal conditions, using 2 mmol/L HAc + 2 mmol/L NaAc (pH 4. 5) as buffer solution, with 0.20 mmol/L SDS as addition at the separation voltage of 2.00 kV and 10s injection, matrine was separated and detected within 2 min. The linear dependence of the concentration of matrine ranged from 10 to 300 microg/mL (r2 = 0.9992), with the detection limit of 1.0 microg/mL (S/N = 3), the RSD of 1.9% and the recoveries from 98.7% to 101%.The method is simple, rapid and well reproducible, and it can be used in the quality control of the product.