Active commuting is associated with a reduced risk of several chronic diseases, but few studies have considered institutional factors that influence it. We examined the association between cultural and physical workplace supports for active commuting and employee active commuting behavior.Data were collected from employees (N = 375) in Manhattan, Kansas, via an online survey. Physical and cultural supports for active commuting in the workplace were measured separately. Active commuting frequency was dichotomized as 0 trips versus at least 1 trip per week by foot or bicycle. Binomial logistic regression was used to predict the likelihood of commuting actively at least once per week, according to the number and type of cultural and physical workplace supports. Analyses were conducted by sex and age and for the full sample.Among the full sample, employees who reported more physical and cultural supports in the workplace for active commuting were more likely to walk or bicycle to work at least once per week. Significant, positive relationships between cultural and physical supports and active commuting were observed for women but not for men. Both younger and older adults who reported 2 or more physical supports in the workplace were more likely to actively commute, but no relationship was observed for cultural supports. The most influential types of individual supports were perceiving that other coworkers actively commute and the presence of bicycle parking and a bicycle storage policy at the workplace.The presence of workplace physical and cultural supports is related to more active commuting behavior and may especially encourage active commuting among women.