To study the chemical constituents from seeds of Paeonia sufruticosa.Various chromatographic techniques were used to isolate and purify the constituents, their physico-chemical properties and spectral data were employed to elucidate their structures.Thirteen compounds were isolated and identified as: paeoniflorin (1), oxypaeoniflorin (2), 6'-O-beta-D-glucopyranosylalbiflorin (3), 8-debenzoylpaeoniflorin (4), 8-debenzoylpaeonidanin (5), 1-O-beta-D-glucopyranosylpaeonisuf-frone (6), 1-O-beta-D-ethyl-mannopyranoside (7), sucrose (8), luteolin (9), apigenin (10), benzoic acid (11) and 1-0-beta-D-(4-hydroxybenzoyl) glucose (12).compounds 2, 4-6 were isolated from this plant for the first time, compounds 7 and 12 were isolated from the family of Paeoniaceae for the first time.