To establish the fingerprint spectrum of the stems of Actinidia deliciosa by HPLC.HPLC fingerprint analysis of the stems of Actinidia deliciosa was developed and the thermo Hypersil BDS C18 column (250 mm x 4.6 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.1% phosphoric acid with gradient elution. The column temperature was 20 degrees C, the detective wavelength was 296 nm, the flow rate was 1.0 mL/min, and the sample injection was 20 microL.Fingerprint spectrum of the stems of Actinidia deliciosa was established, and 16 samples of different origin Actinidia deliciosa were detected. Twenty-eight peaks in chromatogram were common. There was a high similarity and each chromatographic peak was obtained with good separation correlation according to the technical requirements of fingerprint of Chinese traditional medicine.This method is accurate, reliable and provides a scientific basis for controlling the quality of the stems of Actinidia deliciosa.