[Identification and genetic characterization of human enterovirus type 73, 75, and 97 strains of specie B isolated in Shandong province].

Ze-Xin, Tao; Hai-Yan, Wang; Ai-Qiang, Xu; Li-Zhi, Song; Yan, Li; Gui-Fang, Liu; Yao, Liu; Qing-Ying, Fan

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Article . 2010

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[Identification and genetic characterization of human enterovirus type 73, 75, and 97 strains of specie B isolated in Shandong province].

descriptionPublicationkeyboard_double_arrow_right Article 27 Apr 2010Journal:Bing du xue bao = Chinese journal of virology, volume 26 (issn: 1000-8721,

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Authors: Ze-Xin, Tao; Hai-Yan, Wang; Ai-Qiang, Xu; Li-Zhi, Song; Yan, Li; Gui-Fang, Liu; Yao, Liu; +1 Authors

pmid: 20329553

[Identification and genetic characterization of human enterovirus type 73, 75, and 97 strains of specie B isolated in Shandong province].

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Abstract

Molecular typing was conducted for three human enteroviruses (HEV) isolated from acute flaccid paralysis (AFP) cases in Shandong province, China. RNAs from virus supernatants were extracted and complete VP1 genes were amplified by RT-PCR and sequenced. Genotypes of these isolates were identified as HEV type 73, 75 and 97, respectively by BLAST program. Homology and phylogenetic tree analyses were performed. Sequence analysis of VP1 gene showed significant variation compared with prototype strains. This study presents the genetic characteristics of HEV 73, 75 and 97 of specie B in Shandong Province, and the first report of HEV97 in China.

Related Organizations

Shandong Center for Disease Control and Prevention
China (People's Republic of)

Keywords

China, Viral Proteins, Base Sequence, Molecular Sequence Data, Enterovirus Infections, Humans, Paralysis, Phylogeny, Cell Line, Enterovirus

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