To explore the effect of different culture conditions and hormone combinations on callus induction of Ferula sinkiangensis.Using different seedling age of Ferula sinkiangensis young cotyledon, hypocotyl, radicle as explants, which were cultivated on differentiation medium with different hormone combinations, to study the effects on Ferulic callus by different explants and hormone combinations.Ferulic seedlings with no true leaf was more favorable to the callus, the optimum medium for hypocotyl induction was MS + 2,4-D (1.0 mg/L) + KT (1.5 mg/L), for radicle induction was MS + NAA (0.5 mg/L) + 6-BA (0.5 mg/L), and the best subculture medium was MS + NAA (1.5 mg/L) + 6-BA (2.5 mg/L).NAA,2,4-D and 6-BA must have the inductive effects, 6-BA is better than the KT in the process of proliferation and the medium containing GA3 is conducive to the process of subculture embryogenic callus production.