[Determination of chelerythrine in Chelidonium majus by RP-HPLC].
Copyright policy )[Determination of chelerythrine in Chelidonium majus by RP-HPLC].
To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus.Chelerythrine was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine (25:75) (adjusted pH to 3 using phosphoric acid) and the detection wavelength was set at 268 nm.The linear range of calibration curve was 0.051 6-0.516 0 microg (r = 1.000). The average recovery (n = 6) was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns.The method is accurate, sensitive and reliable.
Benzophenanthridines, Chelidonium, Chromatography, High Pressure Liquid, Drugs, Chinese Herbal
Benzophenanthridines, Chelidonium, Chromatography, High Pressure Liquid, Drugs, Chinese Herbal
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