The master's thesis involves the isolation, synthesis and characterization of bacterial nanocellulose. The first part of the master's thesis is focused on the preparation of growth medium and various production media and the cultivation of the bacterium Gluconacetobacter xylinus. In the second part, the isolation of bacterial nanocellulose (BNC) from the calcium carbonate production medium, and the production medium with citric acid by treatment with sodium hydroxide (NaOH) was described. In the end, both BNC samples were characterized by different analytical methods. With the FTIR, SEM and EDX methods was demonstrated that both samples contained cellulose. However, with the EDX/DLS method was observed that BNC obtained from the medium with citric acid is more temperature resistant, and also more BNC have been produced in this medium.

Magistrska naloga zajema izolacijo, sintezo ter karakterizacijo bakterijske nanoceluloze. V prvem delu magistrske naloge smo se osredotočili na pripravo rastnega medija in različnih produkcijskih gojišč ter gojenje bakterije Gluconacetobacter xylinus. V drugem delu smo najprej izolirali bakterijsko nanocelulozo (BNC) iz produkcijskega gojišča z kalcijevim karbonatom ter produkcijskega gojišča s citronsko kislino z obdelavo z natrijevim hidroksidom (NaOH). Na koncu smo oba vzorca BNC karakterizirali z različnimi analiznimi metodami. Z metodami FTIR, SEM in EDS smo dokazali, da sta oba vzorca vsebovala celulozo. Z metodo EDS/DLS pa smo dokazali, da je BNC pridobljena iz medija s citronsko kislino bolj temperaturno obstojna, prav tako se je v tem mediju sintetiziralo največ BNC.