Aim: To explore whether chrysin has any protective effect against the deadly effect of alpha amanitin on hepatocytes that is one of the major toxins in the structure of Amanita phalloides, which is considered the most important mushroom responsible for fatal mushroom poisonings. Materials and methods: For this study, alpha amanitin was purified from A phalloides mushroom using the preparative HPLC (high performance liquid chromatography) method as described in the literature. Four hours after administering alpha amanitin in a concentration of 10 mu g/mL on the cells in a hepatocyte cell line (C3A), silibinin and chrysin were administered in various concentrations. The MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] test was used to determine cell viability. Results: The alpha amanitin was obtained in high purity from the mushrooms and was found to decrease cell viability down to a level of 63% after 48 hours due to its toxic effect on the liver cells in the cell culture. In the groups given silibinin and chrysin, both substances were seen to reduce the toxicity caused by alpha amanitin. Conclusion: Chrysin may play a role in decreasing the tissue damage caused by toxins and lowering the mortality rates in fatal mushroom poisonings associated with alpha amanitin.