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LAReferencia - Red Federada de Repositorios Institucionales de Publicaciones Científicas Latinoamericanas
Article . 2009
License: CC BY NC SA
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Article . 2009
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Horizon / Pleins textes
Other literature type . 2009
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Cryoletters
Article . 2010
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Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi.

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 01 Jan 2009 Argentina, France English Publisher:Cryo LettersJournal:Cryo letters, volume 30 (issn: 0143-2044, Copyright policy )
Authors: Rey, H. Y.; Faloci, M.; Medina, R.; Dolce, N.; Mroyinski, L.; /Engelmann, Florent;

pmid: 19946657

handle: 11336/46756

Cryopreservation of in vitro grown shoot tips and apical meristems of the forage legume Arachis pintoi.

Abstract

A cryopreservation protocol using the encapsulation-dehydration procedure was established for shoot tips (2-3 mm in length) and meristems (0.3-0.5 mm) sampled from in vitro plantlets of diploid and triploid cytotypes of Arachis pintoi. The optimal protocol was the following: after dissection, explants were precultured for 24 h on establishment medium (EM), encapsulated in calcium alginate beads and pretreated in liquid EM medium with daily increasing sucrose concentration (0.5, 0.75, 1.0 M) and desiccated to 22-23% moisture content (fresh weight basis). Explants were frozen using slow cooling (1ºC min-1 from 25ºC to -30ºC followed by direct immersion in liquid nitrogen), thawed rapidly and post-cultured in liquid EM medium enriched with daily decreasing sucrose concentrations (0.75, 0.50, 0.1 M). Explants were then transferred to solid EM medium in order to achieve shoot regeneration, then on Murashige and Skoog medium supplemented with 0.05 μM naphthalene acetic acid to induce rooting of shoots. With this procedure, 53% and 56% of cryopreserved shoot tips of the diploid and triploid cytotypes, respectively, survived and formed plants. However, Orly 16% of cryopreserved meristems of both cytotypes regenerated plants. Using ten isozyme systems and seven RAPD profiles, no modification induced by cryopreservation could be detected in plantlets regenerated from cryopreserved material.

Fil: Faloci, Mirta Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina

Fil: Rey de Badaró, Hebe Yolanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina

Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina

Fil: Dolce, Natalia Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina

Fil: Mroginski, Luis Amado. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina

Fil: Engelmann, Florent. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia

Countries
Argentina, France
Related Organizations
Keywords

encapsulation-dehydration, 570, Sucrose, DNA, Plant, Meristem, shoot tips, In Vitro Techniques, cryopreservation, Genomic Instability, Cryoprotective Agents, https://purl.org/becyt/ford/1.6, apical meristems, Cluster Analysis, Desiccation, Apical Meristems, Genetic Stability, https://purl.org/becyt/ford/1, Encapsulation-Dehydration, Cryopreservation, Fabaceae, stability, genetic, Plant Shoots

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
1
Average
Average
Average
Green