Ion-exchange chromatography.

Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic tool and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.

Keywords

Laboratory Chemicals, Animals, Humans, Chromatography, Ion Exchange, Biochemistry, Protein Binding

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