[MTT assay for detecting osteosarcoma cell apoptosis].
Copyright policy )[MTT assay for detecting osteosarcoma cell apoptosis].
To assess the feasibility of using MTT assay for detecting the apoptosis of osteosarcoma cells following chemotherapy.The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope.MTT staining accurately identified apoptosis of the osteosarcoma cells, and the apoptotic cells were easily distinguished from normal cells and dead cells.MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.
- Southern Medical University China (People's Republic of)
Osteosarcoma, Thiazoles, Antineoplastic Combined Chemotherapy Protocols, Tumor Cells, Cultured, Humans, Tetrazolium Salts, Apoptosis, Bone Neoplasms
Osteosarcoma, Thiazoles, Antineoplastic Combined Chemotherapy Protocols, Tumor Cells, Cultured, Humans, Tetrazolium Salts, Apoptosis, Bone Neoplasms
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