To increase the reproduction efficiency of Iris plants.Pollen viability, stigmatic receptivity, the color of anther and stigma of 5 Iris plants were observed during blooming.1. The highest pollen viability was in 4 hours after blooming; 2. The stigmatic receptivities of I. sichuanensis, I. leptophylla, I. lactea and I. goniocarpa were strong in 4 hours after blooming, while that of I. lactea var. chinensis was strong in 2 hours after blooming; 3. The color of anther could reflect the pollen viability, but could not indicate the viability level; 4. The stigma color could not reflect the receptivity of stigma.The optimum artificial pollination time of these five species were 12:00 -14:00.