The recent research papers in ion-sensitivity in-situ gel were reviewed on the following aspects: the characteristics of the gel, the polymer, preparation technology as well as the applications of optical, nasal, and oral route of administration. The solution-gel property of transformation of in-situ gel, which has simple preparation, convenient operation, strong affinity and enough retention time with affected part, especially with mucosa. The recent research advances in ion-sensitivity in-situ gel were mainly reviewed from the following aspects: the characteristics of the gel, the polymer, preparation technology as well as the applications of optical, nasal, and oral route of administration. The present problems of in-situ gel and prospective applications in traditional Chinese medicine were also discussed.