KCNQ channels play important roles in controlling membrane excitability. A reduction in KCNQ channel activity due to genetic mutation is responsible for various human diseases, including arrhythmia.A combined bioinformatics analysis was performed to study the positions that are at risk for mutation within the amino acid sequence of KCNQ. A new bioinformatics tool, namely GlobPlot, was used.According to the present study, the positions that are resistant to mutation are predicted.Several mutation-prone positions within the KCNQ sequence are reported.