To study the chemical constituents from the flowers of Carthamus tinctorius.Compounds were isolated by chromatographic techniques. Their structures were elucidated by spectral methods.Ten compounds were identified as 7,8-dimethylpyrazino [2,3-g] quinazolin-2, 4-(1H, 3H) -dione (1), adenosine (2), adenine (3), uridine (4), thymine (5), uracil (6), roseoside (7), 4'-O-dihydrophaseic acid-beta-D-glucopyranoside methylester (8), 4-O-beta-D-glucopyranosyloxy-benzoic acid (9) and p-hydroxybenzoic acid (10).Compounds 1 and 8 were isolated from natural plants for the first time, and compounds 7, 9 and 10 were isolated from this plant for the first time.