RNA decay by messenger RNA interferases.
Copyright policy )RNA decay by messenger RNA interferases.
Two abundant toxin-antitoxin (TA) gene families, relBE and mazEF, encode mRNA cleaving enzymes whose ectopic overexpression abruptly inhibits translation and thereby induces a bacteriostatic condition. Here we describe and discuss protocols for the overproduction, purification, and analysis of mRNA cleaving enzymes such as RelE of Escherichia coli and the corresponding antitoxin RelB. In particular, we describe a set of plasmid vectors useful for the detailed analysis of cleavage sites in model mRNAs.
Udgivelsesdato: 2008-null
- University of Southern Denmark Denmark
- University of Newcastle Australia Australia
- Newcastle University United Kingdom
Base Sequence, Escherichia coli Proteins, Bacterial Toxins, RNA Probes, Blotting, Northern, Chromatography, Ion Exchange, RNA, Bacterial, RNA Interference, Codon, DNA Primers, Plasmids
Base Sequence, Escherichia coli Proteins, Bacterial Toxins, RNA Probes, Blotting, Northern, Chromatography, Ion Exchange, RNA, Bacterial, RNA Interference, Codon, DNA Primers, Plasmids
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).22 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!