Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.).
Copyright policy )Cryopreservation by encapsulation-dehydration of plumules of coconut (Cocos nucifera L.).
This study describes the use of an encapsulation-dehydration cryopreservation technique on coconut plumules (apical dome with three or four leaf primordia) excised from embryos. In order to establish a reliable cryopreservation process for plumules, several different key factors were tested: pretreatment duration, sugar concentration, dehydration period and freezing. In parallel, histological studies were performed to describe the structural changes of tissues and plumule cells subjected to dehydration and freezing. A good survival level of around 60% was obtained. However, after 8 months culture regrowth, this level decreased to a maximum of 20 % which was achieved using sucrose treatment. In this paper we report for the first time the regeneration of leafy shoots from coconut plumules after cryopreservation.
Cocos, CULTURE IN VITRO, déshydratation, Sucrose, Cell Survival, http://aims.fao.org/aos/agrovoc/c_27990, CRYOCONSERVATION, http://aims.fao.org/aos/agrovoc/c_3091, F62 - Physiologie végétale - Croissance et développement, plumule, DESHYDRATATION, tissu végétal, PLUMULE, ETUDE COMPARATIVE, ENCAPSULATION, NOIX DE COCO, régénération in vitro, SUCROSE, Cocos nucifera, ANALYSE STATISTIQUE, congélation, http://aims.fao.org/aos/agrovoc/c_24155, Cryopreservation, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_28357, Dose-Response Relationship, Drug, HISTOLOGIE, cryoconservation, CONSERVATION DES RESSOURCES GENETIQUES, http://aims.fao.org/aos/agrovoc/c_27843, Plant Leaves, pousse, Freeze Drying, F02 - Multiplication végétative des plantes, http://aims.fao.org/aos/agrovoc/c_2162, http://aims.fao.org/aos/agrovoc/c_6029, saccharose, http://aims.fao.org/aos/agrovoc/c_14261, encapsulation, http://aims.fao.org/aos/agrovoc/c_7495, http://aims.fao.org/aos/agrovoc/c_34114, conservation biologique, http://aims.fao.org/aos/agrovoc/c_4027
Cocos, CULTURE IN VITRO, déshydratation, Sucrose, Cell Survival, http://aims.fao.org/aos/agrovoc/c_27990, CRYOCONSERVATION, http://aims.fao.org/aos/agrovoc/c_3091, F62 - Physiologie végétale - Croissance et développement, plumule, DESHYDRATATION, tissu végétal, PLUMULE, ETUDE COMPARATIVE, ENCAPSULATION, NOIX DE COCO, régénération in vitro, SUCROSE, Cocos nucifera, ANALYSE STATISTIQUE, congélation, http://aims.fao.org/aos/agrovoc/c_24155, Cryopreservation, http://aims.fao.org/aos/agrovoc/c_1716, http://aims.fao.org/aos/agrovoc/c_28357, Dose-Response Relationship, Drug, HISTOLOGIE, cryoconservation, CONSERVATION DES RESSOURCES GENETIQUES, http://aims.fao.org/aos/agrovoc/c_27843, Plant Leaves, pousse, Freeze Drying, F02 - Multiplication végétative des plantes, http://aims.fao.org/aos/agrovoc/c_2162, http://aims.fao.org/aos/agrovoc/c_6029, saccharose, http://aims.fao.org/aos/agrovoc/c_14261, encapsulation, http://aims.fao.org/aos/agrovoc/c_7495, http://aims.fao.org/aos/agrovoc/c_34114, conservation biologique, http://aims.fao.org/aos/agrovoc/c_4027
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