Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes.

R P, Prioli; J S, Mejia; T, Aji; M, Aikawa; M E, Pereira

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Article . 1991

Data sources: Europe PubMed Central

Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes.

descriptionPublicationkeyboard_double_arrow_right Article 22 Oct 1991 English Journal:Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), volume 42 (issn: 0177-2392,

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Authors: R P, Prioli; J S, Mejia; T, Aji; M, Aikawa; M E, Pereira;

pmid: 1896773

Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes.

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Abstract

Immunoelectron microscopy (IEM) using TCN-2, a monoclonal antibody specific for Trypanosoma cruzi neuraminidase (NA), was performed to determine the precise localization of the parasite enzyme. In agreement with previous observations, TCN-2 reacted with tissue culture trypomastigotes, but not with epimastigotes, amastigotes or intracellular forms in intermediate stages of development. NA was localized on the surface of tissue culture trypomastigotes and in the Golgi apparatus suggesting that the enzyme is modified post-translationally. In agreement with this suggestion, digestion of NA with N-Glycanase, an enzyme that releases N-linked oligosaccharides, decreased the molecular weight of the polypeptides that make up NA.

Related Organizations

Medical Center Hospital
United States

Keywords

Molecular Weight, Glycoside Hydrolases, Trypanosoma cruzi, Cell Membrane, Animals, Antibodies, Monoclonal, Golgi Apparatus, Neuraminidase, Microscopy, Immunoelectron, Protein Processing, Post-Translational

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