
A method was developed for detecting feline leukemia virus (FeLV) infection in cats by coculturing separated blood lymphocytes and platelets with clone 81 cells carrying a murine sarcoma genome but free of leukemia virus (S+L-). FeLV caused cell transformation that was easily visible 3-12 days after infection. This method is simple, sensitive, and easily read; it has the advantage of directly detecting infectious FeLV rather than viral antigens.
Blood Platelets, Leukemia, Leukemia Virus, Feline, Cat Diseases, Cell Line, Tumor Virus Infections, Cell Transformation, Neoplastic, Cats, Methods, Animals, Lymphocytes
Blood Platelets, Leukemia, Leukemia Virus, Feline, Cat Diseases, Cell Line, Tumor Virus Infections, Cell Transformation, Neoplastic, Cats, Methods, Animals, Lymphocytes
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