To evaluate the performances of 3 serological assays (direct agglutination test [DAT], fast agglutination screening test [FAST], recombinant protein [rK39] dipstick) test for use in primary care, for the diagnosis visceral leishmaniasis (VL) in Syria.We utilized 267 serum samples obtained during 2007 from patient groups confirmed and suspected VL, confirmed cutaneous leishmaniasis, toxoplasmosis from endemic areas in Syria and control samples, and applied the 3 serological tests in the Damascus University, Damascus and Health laboratories at the same time, on these samples.Our data show that the tests were very sensitive, where the DAT was the most specific followed by FAST, then rK39 dipstick.Our study confirmed that all the tests performed well, and proved to be very important sero-diagnosis tools for visceral leishmaniasis.