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Horizon / Pleins textes
Other literature type . 2008
Cryoletters
Article . 2009
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Cryopreservation of sugarcane somatic embryos.

Authors: Martinez-Montero, M. E.; Martinez, J.; /Engelmann, Florent;

Cryopreservation of sugarcane somatic embryos.

Abstract

In this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degree C, treatment with the PVS2 solution for 20-40 min at 0 degree C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 percent.

Country
France
Keywords

Cryopreservation, Glycerol, Sucrose, Cell Survival, Water, Sugarcane, Vitrification, 630, Saccharum, Electrolytes, Encapsulation-vitrification, Cryoprotective Agents, Freeze Drying, Droplet-vitrification, Seeds, Somatic embryos

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
4
Average
Average
Average
Green