
In this paper, we compared three vitrification-based cryopreservation techniques, viz. vitrification, encapsulation-vitrification and droplet-vitrification for cryopreserving sugarcane somatic embryos. Viability of somatic embryos was evaluated by measuring electrolyte leakage and by regrowth on recovery medium. Droplet-vitrification was the most efficient technique. Optimal conditions included loading with a solution containing 1.5 M glycerol and 0.3 M sucrose for 30 min at 25 degree C, treatment with the PVS2 solution for 20-40 min at 0 degree C followed by rapid immersion in liquid nitrogen of clumps of somatic embryos placed in microdroplets of cryoprotectant solution. Under such conditions, viability of cryopreserved somatic embryos reached 55 percent.
Cryopreservation, Glycerol, Sucrose, Cell Survival, Water, Sugarcane, Vitrification, 630, Saccharum, Electrolytes, Encapsulation-vitrification, Cryoprotective Agents, Freeze Drying, Droplet-vitrification, Seeds, Somatic embryos
Cryopreservation, Glycerol, Sucrose, Cell Survival, Water, Sugarcane, Vitrification, 630, Saccharum, Electrolytes, Encapsulation-vitrification, Cryoprotective Agents, Freeze Drying, Droplet-vitrification, Seeds, Somatic embryos
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