Powered by OpenAIRE graph
Found an issue? Give us feedback
addClaim

Cryopreservation of black chokeberry in vitro shoot apices.

Authors: Daisuke, Kami; Maki, Uenohata; Takashi, Suzuki; Katsuji, Oosawa;

Cryopreservation of black chokeberry in vitro shoot apices.

Abstract

In vitro shoot apices of black chokeberry (Aronia melanocarpa Elliott) were successfully cryopreserved utilizing three methods, namely vitrification, encapsulation-vitrification and encapsulation-dehydration. Encapsulation-dehydration and encapsulation-vitrification, however, seem preferable to vitrification, since the highest respective survival levels of apices (71.1 +/- 2.2 percent and 77.8 +/- 4.4 percent) by both methods were higher than that (60.0 +/- 3.9 percent) by vitrification. In encapsulation-dehydration, the highest survival was achieved when the moisture content of beads was reduced to 19 percent by drying with silica gel for 6 h. In the present study, it was shown that adding 1.0 M glycerol to beads and loading solution during encapsulation-dehydration resulted in high survival (91.7 - 95.0 percent) regardless of lines and polyploids of black chokeberry.

Related Organizations
Keywords

Cryopreservation, Glycerol, Silica Gel, Water, In Vitro Techniques, Silicon Dioxide, Cryoprotective Agents, Freeze Drying, Photinia, Desiccation, Plant Shoots

  • BIP!
    Impact byBIP!
    selected citations
    These citations are derived from selected sources.
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    3
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Average
Powered by OpenAIRE graph
Found an issue? Give us feedback
selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
3
Average
Average
Average
Upload OA version
Are you the author of this publication? Upload your Open Access version to Zenodo!
It’s fast and easy, just two clicks!