House dust contains various organic and inorganic materials. The most important parts of the house dust are dust mites causing allergic diseases such as allergic rhinitis, asthma, atopic dermatitis and seasonal keratoconjunctivitis. In this study, a total of 72 random house dust samples collected from various regions of Eskisehir were examined. The dust samples were examined by the lactic acid precipitation method which was modified from the Spieksma-Boezaman's method. The mite positive house dust rate was 16.67% including Dermatophagoides pteronyssinus, Chortoglyphus arcuatus and Tyrophagus sp. It was concluded that investigation of a greater number of samples would lead to more precise rate of detection of mite positive dust and also that more mite species would be found.