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[Real-time polymerase chain reaction (RT-PCR)].

Authors: Katarzyna, Wiedro; Ewa, Stachowska; Dariusz, Chlubek;

[Real-time polymerase chain reaction (RT-PCR)].

Abstract

The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology, the possibility of analysis and visualization of products during the time of reaction, enabled the progress of highly effective diagnostics applications.

Keywords

Hydrolysis, Molecular Probe Techniques, Polymerase Chain Reaction, Sensitivity and Specificity, Fluorescence, Nucleic Acid Probes, Molecular Diagnostic Techniques, Animals, Humans, Fluorescent Dyes

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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