
The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology, the possibility of analysis and visualization of products during the time of reaction, enabled the progress of highly effective diagnostics applications.
Hydrolysis, Molecular Probe Techniques, Polymerase Chain Reaction, Sensitivity and Specificity, Fluorescence, Nucleic Acid Probes, Molecular Diagnostic Techniques, Animals, Humans, Fluorescent Dyes
Hydrolysis, Molecular Probe Techniques, Polymerase Chain Reaction, Sensitivity and Specificity, Fluorescence, Nucleic Acid Probes, Molecular Diagnostic Techniques, Animals, Humans, Fluorescent Dyes
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