To study the composition of microflora of lower and upper parts of reproductive system in women with tubal-peritoneal sterility.One hundred and forty-five women with tubal-peritoneal sterilitywere examined. Laparoscopy with biopsy of ovaries and endometrium with evaluation of specimens by histology and PCR was performed in all patients. Cervical material, endometrial and ovarian biopsy specimens, and peritoneal fluid were tested by PCR on the presence of genetic material of sexually-transmitted microorganisms. Cervical material was tested for human papillomaviruses (types 16 and 18).Bacteria were isolated from cervical canal specimens in 40% of women. Chlamydia, Ureaplasma, herpes virus type 1 and 2, cytomegalovirus were detected in specimens of cervical canal, endometrium, ovaries and peritoneal fluid in 80%, 38,6%, 34,3%, and 38,46% of women, respectively.In cases of tubal-peritoneal sterility female reproductive system is infected with different pathogenic microorganisms. Growth of microorganisms was detected in 40% of cervical mucosa samples. Intracellular pathogens persist in upper and lower parts of female reproductive system. It was noted that Herpes simplex infection predominated in ovaries.