In the aim of achieving the best possible results in sports, very often various chemical substances are used, which are not present in the organism or they have been present in a considerably smaller quantity. All these substances are used for a fairly long amount of time, in the last few years on a scientific basis. With their activity they stimulate protein metabolism, and promote the function of the central nervous system. The harmful activity of doping substances is serious with early and late consequences. Proof by chromatographic methods is certain.