Currently available hepatitis B vaccines belong to the II or III generation. II generation vaccines include preparations containing recombinant HBsAg as a result of expression of only HBsAg coding gene in yeast or in mammalian cells, while to the III generation belong vaccines that contain in addition to HBsAg also other viral proteins: preS1 and preS2. They are presumed to be more immunogenic than II generation vaccines, wchich may be of importance for immunization of people with poorly induced immunological response after vaccination. After single administration of low dose (0.2 microg/ml) of tested vaccines humoral response was obtained only after vaccination with PreS + S vaccine, which may indicate higher dynamics of humoral response generation in case of the III generation vaccine. After vaccination with two doses of PreS+S vaccine the level of humoral response was significantly higher in comparison with S vaccine. It could be speculated that such a vaccine might be useful as an alternative for immunization against hepatitis B persons with poor or without any immunological response after routine vaccination schedule.