[Callus induction and cultivation of Camptotheca acuminata].
Copyright policy )[Callus induction and cultivation of Camptotheca acuminata].
To study the callus induction from leaf, stem segments and stem segments with axillary's bud and the subculture conditions of callus in Camptotheca acuminata.The explants were inoculated into the media of MS with different concentrations of 6-BA, NAA and 2, 4-D by orthogonal experiment.The optimal medium for callus induction was MS with 6-BA 2 mg x L(-1), NAA 2 mg x L(-1) and 2,4-D 2 mg x L(-1). It had better effects in leaf than in other explants. The induction ratio in leaf reached above 80%. The callus subcultured on the medium of MS with 6-BA 1 mg x L(-1) and 2, 4-D 1 mg x L(-1) grew vigorous and more quickly than that in other media. It was loose, friable in consistency and suitable for cell culture.
- Southwest University of Science and Technology China (People's Republic of)
Plant Leaves, Tissue Culture Techniques, Plants, Medicinal, Plant Growth Regulators, Plant Stems, Camptotheca, Culture Media
Plant Leaves, Tissue Culture Techniques, Plants, Medicinal, Plant Growth Regulators, Plant Stems, Camptotheca, Culture Media
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