[Voltage-dependent anion channels (VDAC) on human sperm membrane].
Copyright policy )[Voltage-dependent anion channels (VDAC) on human sperm membrane].
To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
- Jiangsu Province Hospital China (People's Republic of)
Male, Blotting, Western, Testis, Gene Expression, Humans, Voltage-Dependent Anion Channels, Polymerase Chain Reaction, Spermatozoa, Recombinant Proteins, Signal Transduction
Male, Blotting, Western, Testis, Gene Expression, Humans, Voltage-Dependent Anion Channels, Polymerase Chain Reaction, Spermatozoa, Recombinant Proteins, Signal Transduction
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